Template Switching Oligo

Template Switching Oligo - Use this set of oligonucleotides for template switching cdna synthesis and preamplification. The tso (template switch oligo) is an oligo that hybridizes to untemplated c nucleotides added by the reverse transcriptase during. Capillary electrophoresis (ce) was used to quantify primer elongation and template switching products, including concatemers that formed. In conjunction with a template switching oligo (tso), cdna is synthesized with a known sequence of choice attached to the 3′ end. Reverse transcriptase then switches templates and continues replicating to the end of the oligo. Template switching oligonucleotide and capturing oligo (dt) are used to.

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(A) First strand cDNA is initiated by priming with an oligo dT primer
Template Switch Oligo
What Is A Template Switching Oligonucleotide
Fig. S12 Schematic representation of the tested oligodT / TSO
(A) The TS mechanism is used for first strand cDNA synthesis. First, an
SMART technology overview
Template switching oligos (TS oligos, TSOs) for cDNA library
Model of cDNA synthesis that occurs in the absence (A) and presence (B
Schematic for cDNA synthesis by templateswitching. (Step 1) Primer

In conjunction with a template switching oligo (tso), cdna is synthesized with a known sequence of choice attached to the 3′ end. Template switching oligonucleotide and capturing oligo (dt) are used to. Use this set of oligonucleotides for template switching cdna synthesis and preamplification. Reverse transcriptase then switches templates and continues replicating to the end of the oligo. Capillary electrophoresis (ce) was used to quantify primer elongation and template switching products, including concatemers that formed. The tso (template switch oligo) is an oligo that hybridizes to untemplated c nucleotides added by the reverse transcriptase during.

Capillary Electrophoresis (Ce) Was Used To Quantify Primer Elongation And Template Switching Products, Including Concatemers That Formed.

In conjunction with a template switching oligo (tso), cdna is synthesized with a known sequence of choice attached to the 3′ end. The tso (template switch oligo) is an oligo that hybridizes to untemplated c nucleotides added by the reverse transcriptase during. Reverse transcriptase then switches templates and continues replicating to the end of the oligo. Template switching oligonucleotide and capturing oligo (dt) are used to.

Use This Set Of Oligonucleotides For Template Switching Cdna Synthesis And Preamplification.

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